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KMID : 0357419990290020099
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Korean journal of Virology
1999 Volume.29 No. 2 p.99 ~ p.105
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Detection of Glycoproteins (B and D) and Ttymidine Kinase Genes of Herpes simplex virus Type 2 Strain G
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Hyun Kang
Jong Kuk Park/Hong Sun Uh/Soo Young Kim/Hyung Hoan Lee
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Abstract
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BamHI restriction pattern and genomic library of Herpes simplex virus type 2 (HSV-2) strain G were constructed, and locations of the glycoproteins gB and gD, and tk genes on the fragments were detected by Southern blot analysis. HSV-2 genomic DNAs were cleaved into twenty-seven fragments by BamHI enzyme in the range of 0.72 to 15.08 (total 150.44 kb), which were cloned into the BamHI site of pBluescript SK(+) to construct genome library of the HSV-2. The library was named by the order of the fragment size from smallest one to largest one. HSV-2 glycoprotein gD gene was located in pHLA2-21 and pHLA2-22 recombinant plasmids, gB gene in pHLA2-24 plasmid, and tk gene in pHLA2-11 clone by Southern blot analysis.
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KEYWORD
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Herpes simplex virus, Glycoprotein, Glycoproteins B and D genes, Thymidine kinase gene, TK protein,
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